Composite
rbs + sam5

Part:BBa_K238007:Design

Designed by: Annelien Verfaillie   Group: iGEM09_KULeuven   (2009-07-27)


Sam5 gene with RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 37
    Illegal NgoMIV site found at 45
    Illegal NgoMIV site found at 795
    Illegal NgoMIV site found at 968
    Illegal NgoMIV site found at 1370
    Illegal NgoMIV site found at 1374
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 569


Design Notes

the PstI restriction site was mutated out.


Source

lab of Prof. C. French of the University of Edinburgh

References